c cell culture human embryonic kidney hek 293t cells (ATCC)
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c cell culture human embryonic kidney hek 293t cells
C Cell Culture Human Embryonic Kidney Hek 293t Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38021 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+cell+culture+human+embryonic+kidney+hek+293t+cells/293T/us12161709-1289-59-68
Average 99 stars, based on 38021 article reviews
C Cell Culture Human Embryonic Kidney Hek 293t Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38021 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+cell+culture+human+embryonic+kidney+hek+293t+cells/293T/us12161709-1289-59-68
Average 99 stars, based on 38021 article reviews
c cell culture human embryonic kidney hek 293t cells - by Bioz Stars,
2026-09
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Modification:Article Title: Nucleoside-modified RNA for inducing an immune response against zika virus Article Snippet: Protein Reagents Purified recombinant ZIKV E protein (Aalto Bioreagents AZ 6312) was used in ELISAs to detect E protein-specific IgG, in Western blots as a positive control and in mouse splenocyte stimulation. mRNA Production mRNA was produced as previously described (Pardi et al, 2013, Methods Mol Biol, 969, 29-42) using T7 RNA polymerase on linearized plasmid (pTEV-ZIKVprM-E-A101) encoding codon-optimized (Thess et al., 2015, Mol Ther, 23: 1456-1464) ZIKV strain H/PF/2013 (GenBank: KJ776791) prM-E glycoproteins. mRNA was transcribed to contain 101 nucleotide-long poly(A) tails. .. 1-methylpseudouridine-5′-triphosphate (TriLink) instead of UTP was used to generate modified nucleoside-containing mRNA. mRNA was capped using the m7G capping kit with 2′-O-methyltransferase to obtain cap1 and was purified by a fast protein liquid chromatography (FPLC) method, as described (Weissman et al., 2013, Methods Mol Biol, 969: 43-54). mRNA was analyzed by agarose gel electrophoresis and stored frozen at −20° Article Title: Compositions for inducing an immune response against zika virus Article Snippet: Protein Reagents Purified recombinant ZIKV E protein (Aalto Bioreagents AZ 6312) was used in ELISAs to detect E protein-specific IgG, in Western blots as a positive control and in mouse splenocyte stimulation. mRNA Production mRNA was produced as previously described (Pardi et al, 2013, Methods Mol Biol, 969, 29-42) using T7 RNA polymerase on linearized plasmid (pTEV-ZIKVprM-E-A101) encoding codon-optimized (Thess et al., 2015, Mol Ther, 23: 1456-1464) ZIKV strain H/PF/2013 (GenBank: KJ776791) prM-E glycoproteins. mRNA was transcribed to contain 101 nucleotide-long poly(A) tails. .. 1-methylpseudouridine-5′-triphosphate (TriLink) instead of UTP was used to generate modified nucleoside-containing mRNA. mRNA was capped using the m7G capping kit with 2′-O-methyltransferase to obtain cap1 and was purified by a fast protein liquid chromatography (FPLC) method, as described (Weissman et al., 2013, Methods Mol Biol, 969: 43-54). mRNA was analyzed by agarose gel electrophoresis and stored frozen at −20° Purification:Article Title: Nucleoside-modified RNA for inducing an immune response against zika virus Article Snippet: Protein Reagents Purified recombinant ZIKV E protein (Aalto Bioreagents AZ 6312) was used in ELISAs to detect E protein-specific IgG, in Western blots as a positive control and in mouse splenocyte stimulation. mRNA Production mRNA was produced as previously described (Pardi et al, 2013, Methods Mol Biol, 969, 29-42) using T7 RNA polymerase on linearized plasmid (pTEV-ZIKVprM-E-A101) encoding codon-optimized (Thess et al., 2015, Mol Ther, 23: 1456-1464) ZIKV strain H/PF/2013 (GenBank: KJ776791) prM-E glycoproteins. mRNA was transcribed to contain 101 nucleotide-long poly(A) tails. .. 1-methylpseudouridine-5′-triphosphate (TriLink) instead of UTP was used to generate modified nucleoside-containing mRNA. mRNA was capped using the m7G capping kit with 2′-O-methyltransferase to obtain cap1 and was purified by a fast protein liquid chromatography (FPLC) method, as described (Weissman et al., 2013, Methods Mol Biol, 969: 43-54). mRNA was analyzed by agarose gel electrophoresis and stored frozen at −20° Article Title: Compositions for inducing an immune response against zika virus Article Snippet: Protein Reagents Purified recombinant ZIKV E protein (Aalto Bioreagents AZ 6312) was used in ELISAs to detect E protein-specific IgG, in Western blots as a positive control and in mouse splenocyte stimulation. mRNA Production mRNA was produced as previously described (Pardi et al, 2013, Methods Mol Biol, 969, 29-42) using T7 RNA polymerase on linearized plasmid (pTEV-ZIKVprM-E-A101) encoding codon-optimized (Thess et al., 2015, Mol Ther, 23: 1456-1464) ZIKV strain H/PF/2013 (GenBank: KJ776791) prM-E glycoproteins. mRNA was transcribed to contain 101 nucleotide-long poly(A) tails. .. 1-methylpseudouridine-5′-triphosphate (TriLink) instead of UTP was used to generate modified nucleoside-containing mRNA. mRNA was capped using the m7G capping kit with 2′-O-methyltransferase to obtain cap1 and was purified by a fast protein liquid chromatography (FPLC) method, as described (Weissman et al., 2013, Methods Mol Biol, 969: 43-54). mRNA was analyzed by agarose gel electrophoresis and stored frozen at −20° Fast Protein Liquid Chromatography:Article Title: Nucleoside-modified RNA for inducing an immune response against zika virus Article Snippet: Protein Reagents Purified recombinant ZIKV E protein (Aalto Bioreagents AZ 6312) was used in ELISAs to detect E protein-specific IgG, in Western blots as a positive control and in mouse splenocyte stimulation. mRNA Production mRNA was produced as previously described (Pardi et al, 2013, Methods Mol Biol, 969, 29-42) using T7 RNA polymerase on linearized plasmid (pTEV-ZIKVprM-E-A101) encoding codon-optimized (Thess et al., 2015, Mol Ther, 23: 1456-1464) ZIKV strain H/PF/2013 (GenBank: KJ776791) prM-E glycoproteins. mRNA was transcribed to contain 101 nucleotide-long poly(A) tails. .. 1-methylpseudouridine-5′-triphosphate (TriLink) instead of UTP was used to generate modified nucleoside-containing mRNA. mRNA was capped using the m7G capping kit with 2′-O-methyltransferase to obtain cap1 and was purified by a fast protein liquid chromatography (FPLC) method, as described (Weissman et al., 2013, Methods Mol Biol, 969: 43-54). mRNA was analyzed by agarose gel electrophoresis and stored frozen at −20° Article Title: Compositions for inducing an immune response against zika virus Article Snippet: Protein Reagents Purified recombinant ZIKV E protein (Aalto Bioreagents AZ 6312) was used in ELISAs to detect E protein-specific IgG, in Western blots as a positive control and in mouse splenocyte stimulation. mRNA Production mRNA was produced as previously described (Pardi et al, 2013, Methods Mol Biol, 969, 29-42) using T7 RNA polymerase on linearized plasmid (pTEV-ZIKVprM-E-A101) encoding codon-optimized (Thess et al., 2015, Mol Ther, 23: 1456-1464) ZIKV strain H/PF/2013 (GenBank: KJ776791) prM-E glycoproteins. mRNA was transcribed to contain 101 nucleotide-long poly(A) tails. .. 1-methylpseudouridine-5′-triphosphate (TriLink) instead of UTP was used to generate modified nucleoside-containing mRNA. mRNA was capped using the m7G capping kit with 2′-O-methyltransferase to obtain cap1 and was purified by a fast protein liquid chromatography (FPLC) method, as described (Weissman et al., 2013, Methods Mol Biol, 969: 43-54). mRNA was analyzed by agarose gel electrophoresis and stored frozen at −20° Agarose Gel Electrophoresis:Article Title: Nucleoside-modified RNA for inducing an immune response against zika virus Article Snippet: Protein Reagents Purified recombinant ZIKV E protein (Aalto Bioreagents AZ 6312) was used in ELISAs to detect E protein-specific IgG, in Western blots as a positive control and in mouse splenocyte stimulation. mRNA Production mRNA was produced as previously described (Pardi et al, 2013, Methods Mol Biol, 969, 29-42) using T7 RNA polymerase on linearized plasmid (pTEV-ZIKVprM-E-A101) encoding codon-optimized (Thess et al., 2015, Mol Ther, 23: 1456-1464) ZIKV strain H/PF/2013 (GenBank: KJ776791) prM-E glycoproteins. mRNA was transcribed to contain 101 nucleotide-long poly(A) tails. .. 1-methylpseudouridine-5′-triphosphate (TriLink) instead of UTP was used to generate modified nucleoside-containing mRNA. mRNA was capped using the m7G capping kit with 2′-O-methyltransferase to obtain cap1 and was purified by a fast protein liquid chromatography (FPLC) method, as described (Weissman et al., 2013, Methods Mol Biol, 969: 43-54). mRNA was analyzed by agarose gel electrophoresis and stored frozen at −20° Article Title: Compositions for inducing an immune response against zika virus Article Snippet: Protein Reagents Purified recombinant ZIKV E protein (Aalto Bioreagents AZ 6312) was used in ELISAs to detect E protein-specific IgG, in Western blots as a positive control and in mouse splenocyte stimulation. mRNA Production mRNA was produced as previously described (Pardi et al, 2013, Methods Mol Biol, 969, 29-42) using T7 RNA polymerase on linearized plasmid (pTEV-ZIKVprM-E-A101) encoding codon-optimized (Thess et al., 2015, Mol Ther, 23: 1456-1464) ZIKV strain H/PF/2013 (GenBank: KJ776791) prM-E glycoproteins. mRNA was transcribed to contain 101 nucleotide-long poly(A) tails. .. 1-methylpseudouridine-5′-triphosphate (TriLink) instead of UTP was used to generate modified nucleoside-containing mRNA. mRNA was capped using the m7G capping kit with 2′-O-methyltransferase to obtain cap1 and was purified by a fast protein liquid chromatography (FPLC) method, as described (Weissman et al., 2013, Methods Mol Biol, 969: 43-54). mRNA was analyzed by agarose gel electrophoresis and stored frozen at −20° Cell Culture:Article Title: Nucleoside-modified RNA for inducing an immune response against zika virus Article Snippet: Protein Reagents Purified recombinant ZIKV E protein (Aalto Bioreagents AZ 6312) was used in ELISAs to detect E protein-specific IgG, in Western blots as a positive control and in mouse splenocyte stimulation. mRNA Production mRNA was produced as previously described (Pardi et al, 2013, Methods Mol Biol, 969, 29-42) using T7 RNA polymerase on linearized plasmid (pTEV-ZIKVprM-E-A101) encoding codon-optimized (Thess et al., 2015, Mol Ther, 23: 1456-1464) ZIKV strain H/PF/2013 (GenBank: KJ776791) prM-E glycoproteins. mRNA was transcribed to contain 101 nucleotide-long poly(A) tails. .. 1-methylpseudouridine-5′-triphosphate (TriLink) instead of UTP was used to generate modified nucleoside-containing mRNA. mRNA was capped using the m7G capping kit with 2′-O-methyltransferase to obtain cap1 and was purified by a fast protein liquid chromatography (FPLC) method, as described (Weissman et al., 2013, Methods Mol Biol, 969: 43-54). mRNA was analyzed by agarose gel electrophoresis and stored frozen at −20° Article Title: Compositions for inducing an immune response against zika virus Article Snippet: Protein Reagents Purified recombinant ZIKV E protein (Aalto Bioreagents AZ 6312) was used in ELISAs to detect E protein-specific IgG, in Western blots as a positive control and in mouse splenocyte stimulation. mRNA Production mRNA was produced as previously described (Pardi et al, 2013, Methods Mol Biol, 969, 29-42) using T7 RNA polymerase on linearized plasmid (pTEV-ZIKVprM-E-A101) encoding codon-optimized (Thess et al., 2015, Mol Ther, 23: 1456-1464) ZIKV strain H/PF/2013 (GenBank: KJ776791) prM-E glycoproteins. mRNA was transcribed to contain 101 nucleotide-long poly(A) tails. .. 1-methylpseudouridine-5′-triphosphate (TriLink) instead of UTP was used to generate modified nucleoside-containing mRNA. mRNA was capped using the m7G capping kit with 2′-O-methyltransferase to obtain cap1 and was purified by a fast protein liquid chromatography (FPLC) method, as described (Weissman et al., 2013, Methods Mol Biol, 969: 43-54). mRNA was analyzed by agarose gel electrophoresis and stored frozen at −20° |